Toxigenic potential of Aspergillus flavus cultures isolated from wheat grains
2016
Krulj, Jelena | Bočarov-Stančić, Aleksandra | Krstović, Saša | Kojić, Jovana | Đisalov, Jovana | Brlek, Tea | Bodroža-Solarov, Marija
As the result of effects arising from climate changes, global trade and technological changes in the processing industries, high emergence of mycobiota and mycotoxins in small grains presents a serious health risk for both humans and animals. The occurrence and frequency of Aspergillus flavus on grain samples of Triticum aestivum ssp. vulgare was investigated during the wheat harvest period in 2015. The incidence of A. flavus amounted up to 2% of wheat samples, with the frequency of 40%. TLC and HPLC were applied in order to determine toxigenic profiles of A. flavus isolates from wheat grains. Qualitative detection of the aflatoxin B1 (AFB1) producing ability was examined by TLC, while quantitative determination of AFB1 was done by HPLC. The results of this investigation showed that two isolates of A. flavus (12 and 201) had the toxigenic potential for the AFB1 biosynthesis after 14 and 21 days of cultivation on Yeast Extract Sucrose Agar (YESA) at 25°C in dark. The present study indicates the need for continuous monitoring of the aflatoxin producing abilities and emergence of A. flavus in the field, as well as during storage and processing of wheat grains.
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