Anti-inflammatory effects of fruit and leaf extracts of Lycium barbarum in lipopolysaccharide-stimulated RAW264.7 cells and animal model
2019
Bae, S.M., Chungnam National University, Daejeon, Republic of Korea | Kim, J.E., Chungnam National University, Daejeon, Republic of Korea | Bae, E.Y., Chungnam National University, Daejeon, Republic of Korea | Kim, K.A., Chungnam National University, Daejeon, Republic of Korea | Ly, S.Y., Chungnam National University, Daejeon, Republic of Korea
Purpose Medicinal herbs have recently attracted attention as health beneficial foods and source materials for drug development. Recent studies have demonstrated that extracts of Lycium's fruits and roots have a range of physiologically active substances. The extract of Lycium's leaves has been reported to have excellent anti-oxidant and anti-microbial activity, but its anti-inflammatory efficacy is not known. The chlorophyll present in the leaves can act as an anti-oxidant or pro-oxidant depending on the presence of light. Therefore, this study analyzed the anti-inflammatory effects of Lycium's fruit extract (LFE), leaf extract (LLE), and leaf extract with chlorophyll removal (LLE with CR). Methods This study examined the inhibitory effects of LFE, LLE, and LLE with CR on pro-inflammatory mediator production as well as on the expression of iNOS and COX-2 in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and BALB/c mice. Results LFE, LLE, and LLE with CR inhibited the production of pro-inflammatory mediators (NO, TNF-¥á, IL-6, and IL-1¥â) and the expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells in a dose-dependent manner. Furthermore, the administration of LLE and LLE with CR inhibited the serum pro-inflammatory cytokine levels and suppressed DNA damage in BALB/c mice. In particular, LLE with CR exhibited the highest anti-inflammatory activity. Conclusion These results suggest that the fruit and leaves of Lycium are potential therapeutic agents against inflammation.
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