Screening for BBTV resistance and molecular characterization of gamma irradiated putative mutants of abaca using gene-specific markers
2015
Descalsota, G.I.L. | Lalusin, A.G. | Dela Viña, C.B. | Mendioro M.S. | Dizon, T.O.
Screening for virus resistance and molecular characterization of 54 abaca lines generated from unirradiated and gamma-irradiated shoot tips of two abaca cultivars, Tinawagan Pula (TP) and Tangongon (TG) were conducted. Phenotyping for bunchy top, bract mosaic and mosaic diseases and PCR-based diagnosis for bunchy top have identified 14 gamma-irradiated TP lines (2,5,6,7,10, 12, 17, 19, 20, 21, 24, 26, 28, and 29) and two gamma-irradiated TG lines (37 and 53) as potential virus-resistant lines. Simple Sequence Repeat (SSR) and Resistance Gene Analogue (RGA) markers were used for molecular characterization. Eight out of 10 SSR markers and one out of two RGA markers tested produced amplification products. A total of 23 alleles and 18 banding patterns were detected using the 9 markers. The number of alleles per marker ranged two to five. Two alleles and 7 banding patterns not found in unirradiated TP were found in the gamma-irradiated TP plants while alleles and 7 banding patterns not found in unurradiated TG plants were found in the gamma-irradiated TG plants. The average Polymorphism Information Content (PIC) of 0.48 was obtained in both the gamma-irradiated TP plants and the unirradiated TP plants. However, a higher PIC value (0.54) was observed in the gamma-irradiated TG plants as compared with a lower PIC value of unirradiated TG plants (0.41). The increase in the PIC value is indicative of the higher genetic variability in the gamma-irradiated abaca plants created by exposure of gamma irradiation.
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