Effect of Aminoglycoside Antibiotics on Mitosis Pathology in Allium-test

The research work examines the effects of aminoglycoside antibiotics (gentamicin, streptomycin, canamicin, amycatin) and cytokinin 6-BAP on the pathology of mitosis in Allium–Test. Research methods: Allium–test, cytogenetic analysis, statistical analysis. Test results of aminoglycoside antibiotics showed an increase of 1.3–3.0 times pathological mitosis in the cells of meristematic tissue of onion roots compared to control in variants of gentamicin in concentrations of 50.0 and 100.0 mg/l and streptomycin — 150.0 and 1000.0 mg/l. At the same time, the use of kanamycin in concentration of 100.0 and 1000.0 mg/l and amicacin of 500.0 mg/l inhibits pathological processes in cells. When considering phase indices, the induction of aminoglycoside antibiotics of mitosis pathology such as mitosis delay in metaphase is noted, which is related to damage to the mitotic apparatus. In the examination of phase indices, induction of aminoglycoside antibiotics of mitosis pathology such as delay of mitosis in metaphase, which is associated with damage to the mitotic apparatus. The most significant changes in the passage of all major phases of mitosis were recorded in the variant ‘streptomycin, 1000 mg/l’. With the simultaneous use of gentamicin and 6-BAP (at a concentration of 5.0 mg/l) an increase in cells has been revealed with mitosis pathology compared to the corresponding variants, when these substances were used separately. The opposite result was noted in the case of the joint use of cytokinin and streptomycin, when the number of cells with mitosis pathology decreased. The data obtained indicate that the tested aminoglycoside antibiotics, primarily streptomycin at a concentration of 1000.0 mg/l, are able to change the rate of entry of the onion cells common into mitosis and the normal flow of fission processes.