A proteomics approach highlights a myriad of transporters in the Arabidopsis thaliana vacuolar membrane
2007
Jaquinod, Michel | Villiers, Florent | Kieffer-Jaquinod, Sylvie | Hugouvieux, Véronique | Bruley, Christophe | Garin, Jérôme | Bourguignon, Jacques
To better understand plant vacuolar functions and identify new transporters present on the tonoplast, a proteomic work was initiated on Arabidopsis thaliana. A procedure was developed to prepare highly purified vacuoles from protoplasts isolated from Arabidopsis cell cultures, and a proteomics approach was designed to identify the protein components present in both the membrane and soluble fractions of the vacuoles. This procedure allowed the identification of 650 proteins, 2/3 of which copurify with the hydrophobic membrane fraction and 1/3 with the soluble fraction. With regard to function, only 20% of the proteins identified were previously known to be associated with vacuolar activities
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por National Institute for Agricultural Research