Validacion del radioinmunoensayo para la estimacion de testosterona, 11-cetotestosterona y 17β-estradiol en plasma de lubina (Dicentrarchus labrax L.)
1986
Prat, F. | Carrillo, M. | de Mones, A.
In the last years, radioimmunoassay (R.I.A.) has been used in order to measure plasma steroid levels. The present work validates R.I.A. for determination of testosterone, 11-ketotestosterone and 17 beta -estradiol in the plasma of sea bass. Prior assay and to increase the assay specificity, the plasma steroids were extracted and purified on Sephadex LH-20 columns. The recoveries (testosterone: 85.2%, 11-ketotestosterone: 88.7%, and 17 beta -estradiol: 71.6%) after chromatography were satisfactories. Precipitation with PEG was used to separate the steroid-antibody complex from free steroid, showing very low levels of non-specific counts. The assay showed a good sensibility, specificity and precision; its accuracy demonstrated that R.I.A. is a valid technic for the measurement of plasma testosterone, 11-ketotestosterone and 17 beta -estradiol in the sea bass
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