Spectrophotometric determination of total alkaloids in some Iranian medicinal plants
2010
Fazel, Shamsa | Hamidreza, Monsef | Rouhollah, Ghamooshi | Verdian-Rizi, Mohammadreza
A simple spectrophotometric method based on the reaction with Bromocresol Green (BCG) was developed for determination of total alkaloids in medicinal plants. A yellow complex forms and is easily extractable by chloroform at pH 4.7. The absorbance of the complex obeys Bear 's law over the concentration range of 4-13 μg atropine per mL of chloroform. This procedure can be carried out in the presence of other compounds without interference.
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