EFFICACY OF ANTIMICROBIAL AGENTS ON IN VITRO MICROPROPAGATION POTENTIAL OF POTATO
2013
Venkatasalam, E P | Pandey, K K | Singh, B P | Thakur, Vandana | Sharma, Shilpa | Sood, Richa | Sharma, Ashwani k
An experiment was conducted to find out the effect of different concentrations of antimicrobial agents viz., carbenicillin, streptocycline, cephotaxime and Plant Preservative Mixture (PPMTM; US patent no. 5,750,402) on in vitro growth and vigour of micro-plantlets of three potato varieties viz. Kufri Chandramukhi, Kufri Chipsona-3 and Kufri Sindhuri. The study revealed that antimicrobial agents, their different concentrations, potato varieties and their interaction had significant effect on different morphological characters viz., microplant height, inter-nodal length, number of leaves, nodes and roots, root length and fresh as well as dry weight. In general, carbenicillin upto 100 mg/l, cephotaxime upto 200 mg/l and PPM upto 0.15% had positive effect on all the morphological characters in all the three potato varieties except microplant root length in Kufri Chipsona-3 and Kufri Sindhuri as compared to control. Therefore, antimicrobial agents’ like carbenicillin upto 100 mg/l, cephotaxime upto 200 mg/l and PPM upto 0.15% can be used for controlling the in vitro microbial contamination without affecting the growth and vigour of micro-plantlets.
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