In vitro responses of water lily Nymphaea lotus to the growth regulators kinetin and indole-3-butyric acid.
2001
Sulaiman E. | Rashid A. A. | Aziz M. A. | Kamarudin M. S.
A protocol for in vitro shoot production was investigated using axillary buds obtained from tubers of Nymphaea lotus. Effects of kinetin (0, 1.0, 2.5, 5.0 and 10 mg/L) and IBA (0, 0.5 and 1.0 mg/L) were examined. Kinetin at 2.5 mg/L either alone or in combination with 1.0 mg/L IBA and kinetin at 5.0 mg/L in combination with 0.5 mg/L IBA resulted in a high proliferation of shoots. Increasing the kinetin concentration to 10 mg/L, at all levels of IBA, reduced the number of shoots produced and the shoots were apparently stunted.
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