Cloning and sequencing of a full length outer membrane protein (omp) gene of Candidatus liberibacter asiaticus causing citrus greening disease
2008
Lau, H. Y. | Habibuddin H. | Zamri I. | Tan, C. S.
Citrus Greening is one of the most serious diseases affecting citrus cultivation in many countries including Malaysia. The disease is caused by a non-cultured, phloem-restricted bacterium-like organism, Candidatus Liberibacter asiaticus. Its uncliiturable and the low concentration in the infected plants make it difficult to obtain sufficient amount of the pathogen to be used as antigen in the production of its antibodies. Recombinant protein of its outer membrane protein (omp) can be used as the antigen to produce the antibodies against this Greening disease. In this study, the gene encoding the omp of the organism was amplified using the total DNA extracted from disease infected citrus midribs. The amplified PCR fragment of the gene (2.3 kb) was then purified, restriction enzyme digested and ligated into a bacterial expression plasmid vector, pRSET B. The construct was verified by restriction enzyme and DNA sequencing. This full length gene of omp will be used in our subsequent studies to obtain the recombinant protein.
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