Construction of plant expression vector for biopharming of nipah virus surface glycoprotein
2008
Tan, C. S. | Gan, K. S. | Wee, C. Y.
In the process of producing Nipah Virus (NiV) vaccine derived from plant, a gene cassette of Nipah virus glycoprotein in plant expression vector was constructed. The Nipah Virus surface glycoprotein gene was obtained by RT-PCR using total RNA from virus culture lysate as a template. The 1.8 kb PCR amplified G gene was then cloned together with cMyc and 6xHis fusion tag at N-terminal into an intermediate vector to obtain CaMV 35Spromoter at 5 '-end and Nos Poly-A terminator at 3 '-end. After the verification by restriction enzymes and DNA sequencing, the gene cassette was further sub-cloned into pCAMBIA2300 vector. The constructed plasmid was then transformed into Agrobacterium tumefaciens strain LBA4404 and was now ready for plant transformation
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