Improvement of antifungal activity of citronella oil against aspergillus flavus on rubberwood (Hevea brasiliensis) using heat curing
2016
Jantamas, S. | Matan, N. | Aewsiri, T.
Optimisation of the inhibitory effect of citronella oil (10, 30 and 50 µg mL-1) with heat curing (30, 90 and 150 °C) and dryingperiods (1, 12 and 24 hours) against a major mould (Aspergillus flavus) found on the surface of rubberwood was investigated using response surface methodology. Specimens were incubated at 25 °C in 100% relativehumidity for 90 days and individually rated for the period it took to achieve zero mould growth onrubberwood. Citronella oil components were analysed by gas chromatography–mass spectrometry. Citronellaoil (50 µg mL-1) with heat curing (30 and 90 °C) and drying periods (1 to 24 hours) completely inhibitedspore germination for at least 90 days. Microscopy investigation confirmed that no spore germinationwas found in treated rubberwood. Citronellal (27.5%), geraniol (20.4%), citronellol (13.4%) were majorconstituents of citronella oil. Heat curing may be important for transformation of components andenhancement of antifungal activity of citronella oil. This study showed that a combination of citronella oiland heat curing could protect rubberwood.
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