Influence of different 2,4-D concentrations on antioxidant contents and activities in sweet basil leaf-derived callus during proliferation
2015
Wongsen, W. | Bodhipadma, K. | Noichinda, S. | Leung, D. W. M.
The potential of sweet basil callus cultures as a source of natural antioxidants was investigated. Sweet basil (Ocimum basilicum L.) leaves were cultured on a semi-solid nutrient medium supplemented with 0, 0.5, 1 or 2 mg/l 2,4-D under a light illumination regime (20 μmol m-2 s-1 for 16 h and 8 h in the dark daily) at 25°C for 4 weeks. All the media used, except the plant growth regulator-free medium, could induce formation of green compact callus. After 4 weeks, callus cultured on the medium supplemented with 0.5 mg/l 2,4-D had the highest fresh weight. Antioxidant content analysis showed that this callus culture had the highest levels of β-carotene, ascorbic acid, phenolics and flavonoids (0.08 mg/gFW, 0.64 mg/gFW, 6.54 mg gallic acid/gFW and 7.38 mg rutin/gFW, respectively) after 1 week of callus proliferation. An evaluation of antioxidant ability suggested that changes in reducing power, ascorbic acid content, DPPH free radical scavenging activity, phenolics and flavonoids contents in sweet basil leaf-derived callus might all be interlinked.
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