Environment contaminant of Bacillus cereus isolated from ready to eat meat curry collected at various locations in Malaysia

Msarah M. J.; Ali A. E.; Sahilah Abd. Mutalib

Environment contaminant of Bacillus cereus isolated from ready to eat meat curry collected at various locations in Malaysia

2017

Msarah M. J. | Ali A. E. | Sahilah Abd. Mutalib

The aim of the study was to isolate and identify Bacillus cereus from meat curry and to subtype the isolated B. cereus using RAPD-PCR and antibiotic resistance pattern. Ready to eat (RTE) meat curry samples purchased from 12 different restaurants at Kajang, Serdang and KL Sentral regions located in Selangor and Kuala Lumpur, Malaysia. Twenty-four isolates biochemically identified as B. cereus. Antimicrobial resistance analysis demonstrated that B. cereus isolates were highly resistance to ceftriaxone (100%), vancomycin (87.5%), clindamycin (91.6%) and nalidixic acid (100%). None of the B. cereus isolates were resistance towards ciprofloxacin (100%), streptomycin (91.6%) and chloramphenicol (83.4%). The B. cereus isolates were examined for randomly amplified polymorphic DNA-polymerase chain reaction (RAPDPCR) using primer S30 (5’-GTGATCGCAG-3’) and discriminated into nine profiles. The antimicrobial analysis showed seven patterns and phenotypically less heterogeneous when compared to RAPD-PCR. A total number of nineteen types of B. cereus have produced by a combination of phenotype and genotype methods. These results demonstrated that both typing method provides evidence of the presence of similarity and diversity of the B. cereus strains from RTE meat curry.

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