Culture dependent and independent studies on emerging food-borne pathogens Cronobacter sakazakii, Klebsiella pneumoniae and Enterococcus faecalis in Indian food
2017
Gautam, R. K. | Kakatkar, A. S. | Pansare Godambe, L. | Shashidhar, R.
Emerging food-borne pathogens Cronobacter sakazakii, Klebsiella pneumoniae and Enterococcus faecalis may cause food-borne diseases in neonates, elderly and immunocompromised patients. Data on prevalence of emerging pathogens in food from India is lacking. Sixty food samples were analyzed from local markets in Mumbai for the presence of C. sakazakii, K. Pneumoniae and E. faecalis. Present study shows that incidence of C. sakazakii and K. pneumoniae was highest in sprouts and least in milk (Table 1). Total 35% and 85% of food samples were positive for C. sakazakii and K. pneumoniae respectively. Culture independent species specific PCR based method was better than conventional culture based method in detecting these pathogens. E. faecalis was not detected in any of the food samples. All the isolates of C. sakazakii and K. pneumoniae were resistant to ampicillin. C. sakazakii was also resistant to cephalothin and K. pneumoniae was resistant to many other beta-lactam antibiotics like amikacin, aztreonam and amoxicillin-clavulanic acid. Our results suggest that conventional method should be carried out in combination with molecular methods for the accurate identification of these pathogens. Increase in drug resistance amongst emerging pathogens is also a concern.
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