Biogenic amines analysis in shrimp pastes belacan obtained from the Northern States of Peninsular Malaysia
2018
Ahmad M. | Bahruddin S. | Gulam R. R. A. | Padilah B. | Fazilah A.
Shrimp paste or belacan production is still largely a cottage industry that generated additional income to many homestay operators or family that involve in fisheries. Belacan production was inconsistent depending on the availability of Acetes shrimp. Market shelf life and quality between products varies as small production volume caters for certain places. A study was initiated to determine the biogenic amines content and several physico-chemical parameters in belacan obtained from the Northern region, Malaysia. Biogenic amines in sample wasextracted using 0.1 M acid hydrochloric (HCl), derivatized with dansyl chloride, separated using ODS Hypersil LC-18 column (250 x 4.6 mm, 5 µm particle size) and elucidated for chromatographic analysis using HPLC HITACHI L4250 UV-Vis. Good linearity with square of regression coefficient (r2) ranging from 0.997-0.999 were obtained for putrescine, cadaverine,histamine and tyramine with LOD ranging from 0.13 mg L-1 for tyramine to 0.57 mg L-1 for cadaverine and LOQ of 0.45 mg L-1 for tyramine to 1.89 mg L-1 for cadaverine. About 47% of samples contain histamine below 50 mg kg-1 (ppm) safe limit. The highest histamine level was 143 mg kg-1 (mean, 57.6 mg kg-1). The average of putrescine, cadaverine and tyraminewere 137, 50.1 and 8.64 mg kg-1 with the highest recorded value of 314, 134 and 20 mg kg-1, respectively (n=15). The salt percentage was higher than 17% whereas the moisture was below 40% in 73% of samples. These findings indicate the need to monitor the histamine content inbelacan products for quality and safety assurance.
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