Impact of immobilized β-Glucosidase treatment on sugarcane juice
2018
Verma, A. K. | Gupta, A. | Keerti | Dubey, A.
β-Glucosidase (BGL) from Bacillus subtilis was immobilized in polyacrylamide gel. Increasing the concentration of either acrylamide or bisacrylamide the yield of entrapped BGL was increased. The immobilized BGL showed 14.02% retention activity after storage for 25 days at 30ºC. However, after three times repeatedly use of immobilized enzyme exhibited retention of 8.5% residual activity. Change in physicochemical properties such as viscosity, density and reducing sugar was identified while sugarcane juice treated with free and immobilized BGL. A remarkable decrease in viscosity of the treated sugarcane juice was observed. Though, an increase in reducing sugar was affirmed in free BGL and immobilized BGL. The absorption spectrum of sugarcane juice was also recovered raised by delivering the phenolic. These bioactive phenolic compounds could be retrieved after passing by a suitable adsorbent like charcoal and used as dietary supplements or other medicinal purposes.
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