Direct shoot regeneration on petiole and leaf explants of papaya cv. eksotika using BAP and NAA
2003
Aziz M. A. | Panjaitan S. B. | Rashid A. A. | Saleh N. M.
An experiment was carried out to determine the most suitable concentration of BAP (6-Benzylaminopurine) and NAA (Napthaleneacetic Acid) for direct shoot regeneration from petiole and leaf explants of papaya cv. Eksotika. Explants were cultured on MS (Murashige and Skoog) medium supplemented with 500mgL-1 caseine hydrolysate, 0.2% phytagel and different combinations and concentrations of BAP and NAA. For petiole explant, MS medium containing 1.0 mgL-1 BAP and 0.05 mgL-1 NAA produced the highest percentage of shoot formation (60.24%) and mean number of shoots per explant (9.5), while MS medium containing 0.1 mgL-1 BAP and 0.05 mgL-1 NAA resulted in the highest shoot length (0.69 cm). No shoot formation occurred on MS medium without BAP. With leaf explants, MS medium containing 1.0 gL-1 BAP and 0.05 mgL-1 NAA produced the highest percentage of shoot formation (70.07%) and mean number of shoots per explant (46.5). The treatment also showed significant difference on mean number of shoots formed per explant compared to other treatments including the control. The treatment also resulted in high shoot length (0.48 cm). Shoot formation did not occur on MS medium without BAP.
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