Plant Growth Promoting Rhizobacteria (PGPR) application with different nitrogen fertilizer levels in rice (Oryza sativa L.)
2018
Fallah, Alireza | Gholizadeh, Abdollatif | Mohammadian, Mohammad | Ghaffari, Hamid | Biabani, Abbas
A factorial experiment was carried out through a completely randomised design with four replications in the Rice Research Institute of Iran, Deputy of Mazandaran (Amol), in 2012 in order to study the effect of Plant Growth Promoting Rhizobacteria (PGPR) with different levels of nitrogen fertilizer application on the yield and yield components of rice (Oryza Sativa L.).Treatments of this pot study included three factors; factor A - Ppeudomonas in four levels of control (no bacteria), P. putida-1, P. putida-2 and P. fluorescens (0,3,3 and 3 g, respectively), Factor B - azotobacter chroococcum in two levels: no aotobacter and azotobacter chroococcum (0 and 3 g, respectively), and factor C: nitrogen fertiliser from urea in four levels of 0, 80, 140 and 200 mg N/kg soil in two stages. The experiment was conducted in pot culture and open air environment. Different parameters were studied that included fertile tillers number, shoot dry weight, harvest index, flag leaf chlorophyll content, grain yield and grain nitrogen concentration. According to the analysis results of data variance, all traits except chlorophyll content had significant difference at P<0.01 level in the interaction of three factors of pseudomonas, azotobacter and nitrogen fertiliser level. The highest mean of flag leaf chlorophyll content was observed in treatment of pseudomonas putida-1 in 200 mg nitrogen fertiliser per kg soil of pot (43.48 SPAD number). The highest mean of grain yield was seen in treatment of pseudomonas putida-1 and azotobacter in 200 mg nitrogen fertiliser per kg soil of pot (104.1 g/pot).
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