Biochemical screening for metalworking fluid degraders
2011
Ahmad Lutfi Lukman | Abu Bakar Salleh | Raja Noor Zaliha Raja Abd. Rahman | Mahiran Basri
Metalworking fluid (MWF) is a major scheduled waste in metalworking industry with more than 20 billion L being produced worldwide, annually. The disposal cost of the waste is high due to its oil content. Biodegradation is difficult due to its toxicity and the presence of biocides. Thus, the aim of this study is to perform bioaugmentation using hydrocarbon-utilising bacteria isolated from oil-contaminated environment. Soil and 01 samples were collected from a workshop and boat jetty in Terengganu. Preliminary screening was done via enrichment culture with 10% MWF waste. A total of 17 and 11 bacteria were isolated from the workshop and jetty respectively. Biodegradative ability of these bacteria were qualitatively tested on three types of MWF waste at 10%, 50c: and 100% concentrations, with 0.05% 2,3,5-triphenyl tetrazolium chloride as growth indicator. From a total of 28 isolates tested, 10 bacteria were found to have good growth in 10% MWFs waste, exhibited by the red colour of formazan, a water-insoluble azo compound formed by reduction of tetrazolium salt. However, increasing the concentrations of MWFs waste to 100% was proven to inhibit bacterial growth. Only 8, 7 and 2 strains were able to withstand 100% of MWF1, MWF2 and MWF3 respectively.
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