Interaction of Piper betel extracts and Fusarium oxysporum in culture
1995
Nik Norulaini N.A. | Afizah A. | Mohd Omar A.K.
Extracts from two varieties of Piper betel, "sirih acheh", were used to examine the effect upon the growth of Fusarium oxysporum in culture. The extracts were co-cultured with the fungus on potato dextrose agar medium. Preliminary bioassay results indicated that the extracts exhibited an inhibitory effect on the growth and development of the fungus. Fungus moved at a much slower pace towards the extract compared to its movement away from the extract. Sign of inhibition was present as early as 2 days with the marked difference in the colony development towards and away from the extract. In the absence of extracts, the fungus grew at a rate of approximately 1.35 cm per day for the first four days and slowed down to 0.8 cm per day for the next four days whereby it reached its maximum diameter on the 8th day. In comparison, the fungal growth in the presence of sirih hitam extract was 0.73 cm per day from day 0 to day 5 and diminished to a rate of 0.33 cm per day the next five days. Sirih Acheh have similar effect on F. oxysporum with the initial growth rate for the first five days being 0.72 cm per dayand the next five days slowed to a rate of 0.34 cm per day. Hence there is no significant difference in the effect of the two varieties upon the development of the fungus. In the presence of both extracts, the colony size attained by the tenth day was smaller than that achieved in the absence of extract.
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