Morfometrijske osobine eritrocita pasa i faktori koji na njih utiču | Factor affecting morphometric characteristic of red blood cells in dog

The main goal of this research is to determine the reference ranges of morphometricparameters of red blood cells of dogs (diameter, circumference, surface) and to determine thespecific factors that affect them. This study included 61 dogs of different age, breed andgender that were subjected to preventive or control examinations. Blood samples were collected from the cephalic vein of all dogs. Two blood smears were prepared from each dog,one using Giemsa staining technique and the other using Diff Quick staining technique. Allthe smears were observed under light microscopy at 100x magnification using immersion inthe monolayer zone. In each blood smear of every animal in particular, 50 red blood cellswere analysed, which meant that for each animal 100 red blood cells had been measuredstained in two different staining methods. For each red blood cell three parameters weremeasured. Results of this study showed that the values of diameter, circumference and surfacewere in following ranges: 6,76-6,86 μm for the diameter, 21,1-21,5 μm for circumference36,1-37,1 μm2 for the surface at the confidence interval of 95%. Analysis of the symmetry andflattening of the distribution, visual analysis of frequency distribution and QQ diagram aswell as the results of Kolmogorov-Smirnov test indicate that the values of diameter,circumference and surface of red blood cells are normally distributed. Analyzed factors inthis study, biological (gender, body mass index, age), pathological (presence of illness), andlaboratory (staining technique) showed no significant influence to the observed morphometricparameters. Red blood cells look similar in both staining techniques visualized under themicroscope, without any morphological deviations caused by staining technique. When itcomes to Diff Quick staining technique, a certain amount of samples (6/61, ~10%) showedaverage surface of red blood cells in the range from 42 to 45 μm2, which is above the maximalrange of the values for the surface of red blood cells obtained by Giemsa staining technique(42 μm2), but these differences did not affect the visualization of red blood cells under themicroscope.