?????????????????????????????????????????????????????????? Metarhizium anisopliae (Metchnikoff) Sorokin ?????????????????????????????????????????????????????????????? | Quantitative and qualitative evaluation of green muscadine fungus, Metarhizium anisopliae (Metchnikoff) Sorokin collected by the spore separation machine for development of insect pest biological control agent
2019
Parichat Jamrutsri(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Entomology) | Sopon Uraichuen(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Entomology)
Quantitative and qualitative evaluation of green muscadine fungus, Metarhizium anisopliae (GMF) collected by the spore separation machine was conducted to develop a biological control agent for the control of sugarcane longhorn stem borer (SLSB) Dorysthenes buqueti. The results showed that the number of GMF spores was decreased 29.84 percent after being filtered through the spore separator machine. Virulence and pathogenicity of GMF inoculum was then tested against SLSB larvae with 50-60 percent accumulative mortality after 30 days infection for the both spore suspension tested. Evaluation effect of tested formulations on viability of GMF conidia was hence taken place. After 12 months of storage at room temperature, GMF conidia obtained from spore separation machine mixed with sterile soil and coconut dust in different ratio was observed for its viability after 24 hours of incubation comparing with GMF sample kept without agent. The best formulation (mixing of soil and coconut dust at 3:1 ratio) gave the best performance with 58.47 percent of germination with the virulence SLSB larvae providing 60 percent of accumulative mortality whereas no mortality was observed from conventional product. This study has demonstrated that GMF could be mass produced in large quantity on rice substrate, then conidia separated and kept in suitable agent for at least 12 months with enough viability and pathogenicity for SLSB control
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