??????????????????????????????????????????????????????????? | In vitro selection of water-deficit tolerant callus of banana cv. Kluai Hom Tong
2020
Monthar Wongmaneeroj(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Research and Academic Service Center. Central Laboratory and Greenhouse Complex) | Surak Jamjumrus(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Research and Academic Service Center. Central Laboratory and Greenhouse Complex) | Rongrong Homhual(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Research and Academic Service Center. Central Laboratory and Greenhouse Complex)
The objective of this research was to select callus of banana Kluai Hom Tong tolerated to water deficit by using polyethylene glycol (PEG) 6000 as a selective agent in aseptic conditions. The results showed that the MS medium containing 5 mg/L BA was suitable for inducing the highest number of shoots and initiated callus at the base of shoots. The 0.5 cm calli were cultured on MS medium containing 5 mg/L BA and 0 (control) 5, 10, 15 and 20 percent (w/v) of PEG for 2 months. It was found that MS medium containing BA 5 mg/L without PEG had an average shoot number of 5.1. Number of shoots derived from callus were decreased when they were cultured on MS media containing 5-15 percent PEG. The color of the calli changed to black color and the lethal percentage was 50-60 percent in MS medium with 10-15 percent PEG, while all calli changed to black at the level of 20 percent PEG . After calli were further cultured in the same media for one more month, the lethal percentage was increased. Then, all of the PEG-treated calli were transferred into MS medium containing 5 mg/L BA without PEG. The results showed that the new buds were induced from the survival callus derived from the media with 5-15 percent PEG, while none of the calli from 20 percent PEG developed into plantlet. In conclusion, the use of PEG at the level of 10-15 percent had a tendency to select the water-deficit tolerant callus of banana Kluai Hom Tong at the laboratory level
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