Analytical techniques for determination of mycotoxins in barley, malt and beer: A review
2019
Karim C. Piacentini (Biotechnology Department, University of Sao Paulo (USP), Av. Professor LineuPrestes, 03178-200, Sao Paulo, Brazil) | Marek Pernica (Research Institute of Brewing and Malting, Mostecká 7, 614 00 Brno, Czech Republic) | Sylvie Běláková (Research Institute of Brewing and Malting, Mostecká 7, 614 00 Brno, Czech Republic) | Josef Čáslavský (Brno University of Technology, Faculty of Chemistry, Institute of Chemistry and Technology of Environmental Protection, Purkyňova 118, 61200 Brno, Czech Republic) | Karolina Benešová (Research Institute of Brewing and Malting, Mostecká 7, 614 00 Brno, Czech Republic)
The increasing interest in defining the content of mycotoxins is related to the development of methods for their determination. Several methods of determination have been published. This paper describes analytical procedures such as (a) sample preparation – sampling and homogenization, (b) extraction and purification – solid phase extraction (SPE), a QuEChERS method (acronymic name from quick, easy, cheap, effective, rugged and safe), solid-liquid extraction (SLE) and immuno affinity extraction (IAE). It also provides a review of, (c) instruments, and other analytical methods such as thin layer chromatography (TLC), enzyme linked immune sorbent assay (ELISA) and liquid chromatography (LC), gas chromatography (GC) with different detectors for determination of mycotoxin in barley, malt and beer are discussed.
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