Study and comparative of polymorphism (T487C) in exon 17 of the DGAT1 gene and its relationship with carcass weight and dressing percentage in the Lori-Bakhtiari and Zel sheep breed
2011
Hossin Mohammadi | Mohammad Moradi | Mostafa Sadeghi
Diacylglycerol acyltransferase 1 (DGAT1) is one of the candidate genes to improve carcass characteristics in feedlot animals. Gene product coding, a microsomal enzyme that final triglyceride synthesis, hence converting diacyl glycerol to triglyceride accelerated. The purpose of this research, study area (T487C) in exon 17 of the above gene polymorphisms with carcass traits in two Lori-Bakhtiari and Zel breed. For this purpose, during the 35 period animals randomly slaughtered in the abattoir were recorded. DNA was extracted using salting-out method, 152 samples of Lori-Bakhtiari sheep and 157 samples of Zel sheep were obtained, polymerase chain reaction to amplify 309 bp of exon 17 DGAT1 gene using a pair of specific primers was performed. Genotypes obtained from method PCR-RFLP and directly from agarose gel. In Lori-Bakhtiari sheep in two alleles T and C with frequency of 0.746 and 0.254 respectively, were observed, in population Zel Sheeps the two T and C, with frequency 0.81 and 0.19 respectively, observed. Statistical analysis showed polymorphism in exon 17 region of the gene significantly correlated with carcass weight and dressing percentage (P < 0.05). So that the CC genotypes of the significant mean carcass weight and dressing percentage heavier than had TT genotypes (P< 0.05). Of polymorphism can be observed that improvement in breeding programs to improve carcass weight and dressing percentage through selection in favor of superior genotypes be used.
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