Determination of concentration 2,4-D and 6-BAP to induction morphogenic callus of Sweet Potato
2003
Orlando S. González Paneque | Isabel Milanés Vega | Juan J. Silva Pupo | Angel Espinosa Reyes | Leonardo Acosta Pompa
Tubers from Inivit B 90-1 and Yabú-8, cultivars were put in the laboratories into water bottle and we chose explants of limbs from sprout. Explants were disinfected with Sodium Hypoclorite (Na OCl) with active chlorine (1%) and they were put into Murashige and Skoog culture medium under different concentrations of 2,4-D independent and combined with 6-BAP in the culture medium. Crops were put on the energy illumination. The best results in four weeks at the morfogenic callus induction were obtained with the concentrations of 2,4-D (0.1 mg.l-1) and 2,4-D (0.1 mg.l-1) combined with BAP (0.2 mg.l-1) in the culture medium.Key words: clon, explants, Ipomoea, tubers
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