Development of clove oil based nanoformulation against Fusarium oxysporum
2016
PRITI SABNIS, ANJU KAMRA | SATYAWATI SHARMA, ABHISHEK SHARMA*, GUNJAN SEHRA,
Fusarium oxysporum f. sp. lycopersici, a soil-borne pathogen, causes wilt disease in tomato crop. The fungus has the ability to persist in soil for very long periods without a host. Chemical strategies involving use of synthetic pesticides for the control of fungal wilts have now been discouraged due to undesirable effects on soil health, humans and non-targeted organisms in the environment. The focus is, therefore, shifted to search potent chemicals of biological origin for the management of fungal wilt diseases. In present investigation, the antifungal effects of four essential oils viz., clove (Syzygium aromaticum), lemongrass (Cymbopogon citratus), mint (Mentha x piperita) and eucalyptus (Eucalyptus globulus) oils were evaluated against above mentioned wilt causing fungus. Among the all selected oils, clove oil showed maximum antifungal activity and hence it was selected for the development of oil in water nanoemulsion formulation. The nanoformulation thus obtained (clove oil 10% w/w) was characterized by particle size analyser, Atomic force microscopy and Transmission electron microscopy. The stability test conducted revealed the shelf life of formulation to be two years with MIC of 0.4%.
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