High incidence of lipid deposition in the liver of rats fed a diet supplemented with branched-chain amino acids under vitamin Bsub(6) deficiency
2013
Kaimoto, T. (Shikoku University, Tokushima, Tokushima (Japan). Department of Living Science) | Shibuya, M. | Nishikawa, K. | Maeda, H.
Male Wistar rats were fed four diets composed of purified 20% vitamin-free casein diet with (+) or without (-) vitamin Bsub(6) (7.0 mg of pyridoxine HCl/kg of diet) and with (+) or without (-) branched-chain amino acids (BCAAs) of valine, leucine, and isoleucine (4.75%): Bsub(6)(+)BCAA(-); Bsub(6)(+)BCAA(+); Bsub(6)(-)BCAA(-); and Bsub(6)(-)BCAA(+) for 21 d. Among rats fed the Bsub(6)(-)BCAA(+) diet, about a half showed lipid deposition in the liver. On the other hand, serum triacylglycerol levels in the Bsub(6)(-)BCAA(+) group tended to be decreased. Hepatic triacylglycerol and cholesterol levels tended to increase in the Bsub(6)(-)BCAA(+) group compared with the other three groups. Serum apolipoprotein B and apolipoprotein E (apo E) levels in the Bsub(6)(-)BCAA(+) group were the lowest among the three groups. In contrast, hepatic apo E levels in the Bsub(6)(-)BCAA(+) group were the highest among the three groups. High-performance liquid chromatography of pooled serum of rats with lipid deposits revealed that triacylglycerol and cholesterol levels in very low-density lipoprotein (VLDL) were decreased compared with other diet groups. These results strongly suggest that one of the mechanisms of lipid deposition in rats fed a Bsub(6)(-)BCAA(+) diet is due to impaired secretion of VLDL.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Agriculture, Forestry and Fisheries Research Information Technology Center