Cryopreservation of 'Laguna Tall' coconut (Cocoa nucifera Linn.) mature zygotic embryos
2020
Kamaunang, J. | Damasco, O.P. | Protacio, C.M.
'Laguna Tall' coconut (Cocos nucifera L.) zygotic embryos were successfully cryopreserved through cryoprotection using pretreatment-desiccation or vitrification, rapid freezing in liquid nitrogen, rapid thawing at 40 deg C for 2 min, and recovery and growth of frozen embryos in coconut germination medium. Pretreatment in 0.5 M sucrose for 1 d followed by 24 h desiccation in silica gel gave the highest recovery and complete germination (47%) of cryopreserved embryos. This was followed by 24 h desiccation alone (43%), 3 d sucrose pretreatment + 24 h desiccation (33%), and the least recovery from vitrification using PVS4 solution (13%). Embryo reinvigoration as a post thawing treatment and addition of 0.5 mg/L benzyl amino purine + 1.0 mg/L napthalene acetic acid onto Y3 germination medium hastened recovery and significantly improved complete germination of cryopreserved embryos. The study demonstrated the feasibility of cryopreservation for long-term conservation of coconut genetic resources and for the first time reported on the success in cryopreservation of 'Laguna Tall', an endemic coconut variety grown in the Philippines.
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