The experience of genetic certification of ash using microsatellite markers S.G.

At present, ash (Fraxinus spp.) are exposed to pests and pathogens (including the narrow-bodied ash emerald beetle Agrilus planipennis Fairmaire and the parasitic fungus Hymenoscyphus fraxineus), and therefore their populations in the European part of Russia suffer significant damage. For ash, which is a valuable forest species, in conditions of the threat of population decline, it is relevant to use genetic certification methods. For valuable forest species, it is relevant to use methods of genetic management. This article presents results of genetic certification of common ash (Fraxinus excelsior L.). For the analysis, ten pairs of primers for microsatellite loci were used. The result showed the complete specificity of these markers for the studied samples, and also made it possible to identify significant polymorphism among the studied representatives. Based on the results of PCR analysis, studied loci demonstrate the presence of a wide range of various amplification products, including double and triple ones. There is also a significant number of products differing in weight within one to two tens of nucleotides. Based on these results, it should be assumed that the studied samples are characterized by significant genetic diversity, despite growing in the same territory. As a result of the study, it can be concluded that the primers used are suitable for genotyping local ash representatives. The obtained genetic passports make it possible to demonstrate the profile of the microsatellite loci of the studied samples; they are suitable for their identification and comparison with other ash specimens.