STUDY OF THE EFFECT OF DISINVASION SUBSTANCES ON EYMERIA OOCYSTS IN VITRO
2022
Arzu Badirova
The article deals with the study of the disinfestation effect of solutions of different concentrations of chemicals against Eimeria oocysts of sheep in vitro. Sodium alkali, formalin, and phenol (carbolic acid) were taken from chemicals in order to study the destructive effects of disinvasion substances on sporulated and non-sporulated Eimeria oocysts. During the research, the destructive effects of sodium alkali and formalin for experimental purposes and phenol for comparative purposes on sporulated and non-sporulated Eimeria oocysts were studied in laboratory conditions (in vitro). In laboratory studies, it was determined that 8.0% and 10.0% sodium alkali solutions destroy Eimeria oocysts by 100%. The study of the effect of solutions of different concentrations of disinfestation substances on non-sporulated Eimeria oocysts shows that the 2.0% solution of sodium alkali has 64.3%, the 4.0% solution has 78.6%, and the 6.0% solution has 92.8%. The 8.0 and 10.0% solutions have a 100% destructive effect. During the conducted studies, the 2.0% solution of formalin was 42.8%, the 4.0% solution was 57.1%, the 6.0% solution was 71.4%, and the 8.0% solution was 78.6 %, and the 10.0% solution destroyed 85.7% of unsporulated Eimeria oocysts. For comparison, the 5.0% solution of phenol used had a 78.6% destruction effect. The study of the effect of disinfestation solutions of different concentrations on sporulated Eimeria oocysts shows that the 2.0% solution of sodium alkali has 53.8%, the 4.0% solution has 77.0%, and the 6.0% solution has 84.6% , the 8.0% solution is 92.3%, and the 10.0% solution is 100% destructive. During the conducted studies, the 2.0% solution of formalin was 38.5%, the 4.0% solution was 53.8%, the 6.0% solution was 61.5%, and the 8.0% solution was 69.2%, and the 10.0% solution destroyed 77.0% of the sporulated Eimeria oocysts. For comparison, the 5.0% solution of phenol used was 69.2% effective. Normal water was used for control purposes, and Eimeria oocysts were not destroyed. An 8.0% solution of sodium alkali destroys 91.7% of Eimeria oocysts in a 1 m2 area.
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Este registro bibliográfico ha sido proporcionado por Genetic Resources Institute of Azerbaijan National Academy of Sciences