Optimisation of ultrasound assisted extraction of pomposia (Syzygium cumini L.) anthocyanins and phenolic compounds based on response surface methodology
2020
Morsy, N. F. S. | Abd El-Salama, E. A. | Hammad, K. S. M.
Ultrasound-assisted extraction in combination with stirring was used to extract bioactive compounds from peel and flesh of pomposia (Syzygium cumini L.) fruits. Two independent factors including sonication power (90 - 150 W) and extraction time (10 - 30 min) were studied using 3² full factorial design to achieve optimal yields of both total polyphenols content (TPC) and total monomeric anthocyanins content (TMAC). The experimental data were fitted using reduced-order cubic models. The optimum conditions for simultaneous extraction of TPC and TMAC from flesh and peel were 135.73 W at 30 min and 100.30 W at 30 min, respectively. High performance liquid chromatography (HPLC) analysis was used to identify and quantify the polyphenol components. Comparing IC₅₀ of butylated hydroxytoluene (BHT) (23.19 mg/L) with that of peel and flesh extracts (2.82 and 10.24 mg GAE/L, respectively) indicates the superior scavenging activity of these extracts against DPPH radicals.
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