Formation of protoplasts from Pyricularia oryzae
1985
Lee, Y.H. | Chung, H.S. (Seoul National Univ., Suwon (Korea R.). Dept. of Agricultural Biology)
The optimum conditions of protoplasts formation from Pyricularia oryzae were investigated with lytic enzymes and osmotic stabilizers. The mycelia begun to release the protoplasts in response to the complex enzyme solution after 30-60 minutes and reached to maximum after 2-3 hrs. Among the lytic enzymes tested, the mixture solution containing beta-Glucuronidase (0.01 ml/ml), Cellulase ONOZUKA-RS (20 mg/ml), Driselase (10 mg/ml), and Macerozyme R-10 (10 mg/ml) resulted in the highest rate of protoplasts releasing of Pyricularia oryzae. The best stabilizer was 0.6M KCl at pH 7.0
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