The laboratory response of two maize varieties and three hybrids to two types of aflatoxin-producing Aspergillus strains
1987
Anguiano, R.G.L. | Guzman de Pena, D. (National Polytechnic Inst., Irapuato, Gto (Mexico) | Center for Research and Advanced Studies)
One of the most recent approaches for controlling aflatoxin contamination of maize is the use of resistant maize hybrids. This study investigated the susceptibility in the laboratory of three hybrids and two varieties of maize grown in the Ba1873jio region of Mexico to two aflatoxin-producing strains of Aspergillus. These strains were found in samples of maize in the region; their toxin-producing capacity had been recognized in previous tests. Fifty-gram samples of the maize genotypes were inoculated with a determined number of spores suspended in distilled water, and were incubated at room temperature (25 dg. to 27 dg. C.) for seven days. The methods used for the analysis and quantification of aflatoxin were those of Shotwell et al., Nabney and Nesbitt, and a modified CB test. Despite the small number of materials studied, it was possible to separate the maize genotypes into three groups according to their response, susceptible (good substrates), resistant (poor substrates) and ambiguous, depending on the strain used as the inoc
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