Base composition of the DNA of mycoplasma-like organisms associated with various plant diseases
1989
Kollar, A. (Biologische Bundesanstalt fuer Land- und Forstwirtschaft, Dossenheim (Germany, F.R.). Inst. fuer Pflanzenschutz im Obstbau) | Seemueller, E.
DNA was isolated from periwinkle plants infected by various mycoplasma-like organisms (MLOs), and from apple trees affected by apple proliferation. The DNA of the causal agents was separated from the host plant DNA by repeated bisbenzimide-CsCl buoyant density gradient centrifugation which resulted in highly enriched MLO DNA bands characterized by a lower buoyant density thant that of the host DNA. The MLO DNAs were hydrolyzed to free bases which were determined by HPLC. The analysis revealed a similar low G + C content as found in the DNAs of several culturable mycoplasmas and spiroplasmas. The values of the DNA of the agents of the diseases investigated were as follows: European aster yellows 23.0, periwinkle virescence 23.5, apple proliferation 23.7, rape virescence 24.2, and phyllody of Diplotaxis erucoides 26.2 mol per cent G + C, respectively. Methylated bases were detected in low amounts only
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