Genotyping of beta-lactoglobulin gene in different breeds of cattle in Slovakia
1995
Uhrin, P. | Chrenek, P. | Vasicek, D. | Bauerova, M. | Bulla, J. (Vyskumny Ustav Zivocisnej Vyroby, Nitra (Slovak Republic))
The genetic variants of beta-lactoglobulin are associated with lactation performance and have a major influence on the composition of milk and on its processing properties, including cheese yield. The BB genotype is associated with higher fat and casein content and is more desirable for cheese making. The frequencies of beta-lactoglobulin alleles of the following breeds of cattle were determined: Holstein Friesian, Black Pied Lowland, Simmental and Pinzgau. Analyses were accomplished from the DNA of blood or hair root samples by methodology based on polymerase chain reaction (PCR). Time required for PCR analysis was substantially decreased in our analyses and the changes did not have any effect on the quality of obtained PCR products. The frequencies of B allele were 74 % in Holstein Friesian, 64 % in Black Pied Lowland, 62 % in Simmental and 77 % in Pinzgau breed.
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