Use of in vitro methods for plant genetic conservation
1995
Kovac, J. (Palackeho Univ., Olomouc (Czech Republic). Prirodovedecka Fakulta)
Plant species with recalcitrant seeds and species that do not produce seeds may be conserved ex situ by using in vitro techniques. These primarily involve the use of reduced growth and cryopreservation in liquid nitrogen. Reduced growth storage was used successfully to store cultures in the short and medium terms. Growth reduction is achieved by modifying various parameters, such as temperature, culture medium and gaseous environment. Among the methods of reduced growth storage, reduced incubation temperature storage has been widely applied to a number of temperate crops as well as tropical crops. Recently, a new cryopreservation method termed encapsulation/dehydration/vitrification was presented to simplify the procedures of cryopreservation. Besides of slow growth and cryopreservation there are also important applications of in vitro techniques in other areas, including germplasm collection, multiplication and exchange.
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