The application of random amplified polymorphic dna (rapd) technique for microbial genetic difference determination
1995
Fanedl, L. | Avgustin, G. (Ljubljana Univ. (Slovenia). Biotechnical Fac., Zootechnical Dept.)
The finding that amplification of genomic DNA can be directed by only one oligonucleotide primer of arbitrary sequence to produce a characteristic spectrum of DNA products of varying length and complexity, was recently applied as a strategy to investigate genetic differences among organisms. The application of random amplified polymorphic DNA (RAPD) does not depend on cloning or DNA sequence information and can generate fingerprints from DNA of viral, bacterial, fungal, plant and animal origins. Primers 5-15 nucleotides in length can produce complex banding patterns that can easily be resolved by agarose or polyacrylamide gel electrophoresis. RAPD typing is cheaper, faster and at least as sensitive as other typing methods for discriminating among related strains or even species. The RAPD method proved to be very useful for studies of microbial population genetic structure and evolution, as well as for epidemiological studying. In this review we describe the new DNA polymorphisms assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence.
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