Microbiological diagnosis and drug resistance patterns of infectious mastitis in cattle
1994
Nakavuma, J. | Byarugaba, D.K. | Kitimbo, F.X. | Musisi, L.N. (Makerere University, Kampala (Uganda). Dept. of Veterinary Microbiology and Parasitolology)
A total of 250 cow milk samples were submitted to the Makerere Veterinary Microbiology Diagnostic Laboratory between January 1992 and July 1994 form the area served by the Ambulatory Clinic if the Faculty of Veterinary Medicine, and from other field veterinarians in and around Kampala. These samples were examined microbiologically and tested for drug sensitivity. Bacterial isolation was carried out on routine bacteriological culture media which included blood agar, nutrient agar, and MacConkey agar. Presumptive grouping of the isolates was based on colony characteristics plus heamolytic patterns and confirmed by Gram staining. Isolated pathogens included six isolates of Corynebacterium spp., 3 of Pseudomonas spp, 8 of Streptococcus spp, 47 of Staphylococcus spp, and 67 of Klebsiella spp. There were 28 isolates of coliforms (Escherchia or Enterobacteria spp) and 5 of Candida spp. Sensitivity testing was performed using 10 drugs, some of which are commonly used for mastitis treatment in the area. The organisms were least resistant to drugs that act by inhibition of protein synthesis compared to those that inhibit cell wall synthesis and impair membrane function. Broad spectrum drugs were found most effective, except tetracycline
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