Isolation and characterisation of the proteolytic enzymes of carp hepatopancreas
1997
Kminkova, M. | Moucka, Z. | Kucera, J. (Vyzkumny Ustav Potravinarsky, Prague (Czech Republic))
Proteolytic enzymes (trypsin, chymotrypsin, aminopeptidase and carboxypeptidase A and B) were separated from carp hepatopancreas, purified and partially characterized. Aminopeptidase was separated from other enzymes by gel chromatography on Sephadex G-100. Chymotrypsin and trypsin were separated to two active components (chymotrypsin I and II and trypsin I and II) by ion exchange chromatography on DEAE-cellulose. Isoelectric points, optimum temperature and pH were determined for chymotrypsin I and II, trypsin I and II and carboxypeptidase A and B. The carp enzymes has similar properties to those of other water animals.
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