Influence of media gelling agents on root biomass and in vitro va-mycorrhizal symbiosis of carrot with Gigaspora margarita
1997
Adholeya, A. | Verma, A. | Bhatia, N.P.
An in vitro study with Ri-TDNA transformed roots of carrot (Daucus carota) was carried out to evaluate the role of macro-elements contributed as impurities in the gelling agent (phytagel) over and above those present in the minimal (M) medium. Production of root biomass was taken as a measure to quantity the influence of macro-elements added to the minimal medium. The levels of phosphorus when adjusted to 1.19 mg/l and 1.09 mg/l, lead to dry root biomass production at par with the control. Attempts made to lower the amount to impurities in phytagel by de-ionization using different alkalies, proved NaOH to give the best results in terms of relatively high amount of root biomass. In an in vitro dual culture system with carrot as host and Gigaspora margarita as the vesicular-arbuscular mycorrhizal fungus, phytagel impurities helped to produce maximum number of infection units and auxiliary cells when phytagel was added to the minimal medium
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