Optimization of PRINS and C-PRINS for detection of telomeric sequences in Vicia faba
1998
Kubalkova, M. | Dolezal, J. (Akademie Ved, Olomouc (Czech Republic). Ustav Experimentalni Botaniky)
The purpose of this work was to analyse the effect of factors which influence the specificity and sensitivity of the PRINS, including the age of slides, type of Taq DNA polymerase, number and concentration of primers, the presence and concentration of bovine serum albumine and MgCl2 in the reaction mixture. Furthermore, the effect of various pre-treatments on signal intensity and non-specific fluorescence was studied. We have found that the age of slides was critical and that under optimal conditions it was possible to achieve relatively high signal to noise ratio.
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