Variation and inheritance of isozyme loci in controlled crosses of Salix alba and Salix fragilis
1998
Triest, L. | Degreef, B. | Dhaeseleer, M. | Echchgadda, G. | Vanslycken, J. | Coart, E.
The expression of polymorphism in different enzyme systems was investigated in clones of Salix alba and Salix fragilis. Dormant buds and sprouting leaves were used but only few consistent enzyme patterns could be revealed and wereretained for further testing. When considering all obtained enzyme profiles, a total of 43 genes could be scored, of which 15 may show polymorphism within S. alba or S. fragilis. Polymorphism was observed in aconitase, alcohol dehydrogenase, phosphoglucomutase, leucine aminopeptidase, glutamate oxaloacetate transaminase, beta- esterase, isocitrate dehydrogenase, glutamate dehydrogenase, peroxidase, superoxide dismutase and xanthine dehydrogenase. The genetic basis of phosphoglucomutase, leucine aminopeptidase glutamate oxaloacetate transaminase and shikimate dehydrogenase could be inferred directly from intraspecific and interspecific controlled crosses. Full- sib progeny showed mendelian segregation for lap-1 and most of the pgm-2 alleles, whereas in other genes such as beta- est-1 and adh-2, there was only fixed heterozygosity. Most of the isozymes can not be used directly as good markers at species level. Differences between S. alba and S. fragilis are mainly in the allele frequencies. The number of genes and alleles of the polyploid S. alba and S. fragilis were compared to those of earlier reports on diploid Salix species. The polyploids contain less genetic diversity in their soluble enzymes than the diploids. Duplicatedgenes did not led to a substantial increase in the heterozygosity levels in the S. alba-S. fragilis complex.
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