Genetic characterisation of Populus tremula regions of origin in spain using RAPD fingerprints
2000
Sanchez, N. | Grau, J.M. | Alba, N. | Manzanera, J.A. | Bueno, M.D.
Random Amplified Polymorphic DNA (RAPD) was used to identify 89 Populus tremula trees sampled from different Spanish origins. The combination of four primers provided a set of four banding patterns that permitted the identification of all genotypes with a high discrimination capacity. Some trees located in the same stand or in near places showed the same fingerprint, probably due to clonal propagation. Multidimensional scaling analysis of the amplification bands showed low genetic variability between different Spanish origins, but two different groups could be distinguished. The first one formed by individuals from the Northwest (Leon, Lugo, Orense and Zamora) and Huesca; and the second one by Huesca, Segovia and Navarra. Huesca province (near the Pyrenees, in Northeast Spain) contained both groups, showing high variability between individuals of the same origin. Analysis of the RAPD fingerprints from different origins showed that Huesca had the greatest number of banding patterns in common with others origins, suggesting a genetic flow from the Northeast to the rest of the Iberian Peninsula.
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