Effect of acute consumption Passiflora setacea juice on the monocyte adhesion to endothelial cells: a validation ex vivo study
2015
Duarte, I. | Morand, Christine | Milenkovic, Dragan | Monfoulet, Laurent-Emmanuel | Pineli, | Carvalho Da Costa, A.M.
The pulp of Passiflora setacea (PS), a wild species of Brazilian Savanna, has been recently shown to have higher levels of phenolic compounds, when compared to its commercial species. The leading cause of CVD is atherosclerosis and a diet rich in phenolic compounds may have some effects on monocyte adhesion. Therefore, the aim of the present study was to investigate effect of consumption of PS juice by healthy volunteers on the endothelial cell function, particularly on monocyte adhesion on TNF-alfa-activated endothelial cells.Three middle-aged volunteers were included in a cross-sectional study to analyse the effect of 250mL PS juice consumption. A pool of fasting serum samplings were performed before (t0h) and 3h after the consumption (t3h). Primary human umbilical vein endothelial cells (HUVEC) were cultivated in an endothelial growth medium (EGM) with 25% of human serum (HS) for 7h with a 4-hour-stimulation of 1 ng/mL of TNF-alfa. Monocytes (THP-1) were added and incubated for 15min with HUVEC. The number of THP1 per HUVEC was counted in microscopic fields.In preliminary results, the treatment with TNF-alfa significantly increased cell adhesion compared with unstimulated control cells (a 3.93-fold increase). Pre-exposure of HUVEC to human serum sampled 3 hours after the consumption of PS juice significantly reduced monocyte adherence to TNF-alfa-activated endothelial cells from - 59 to - 43% (P<0,05). The decrease of monocytes adhesion to endothelial cells may indicate an anti-inflammatory and atheroprotective effects of PS juice consumption in healthy volunteers.
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