Performance of a multi-species-plant illumina beadchip
2016
Le Paslier, Marie-Christine | Berard, Aurélie | Chauveau, Aurelie | MARQUAND, ELODIE | Boland-Auge, Anne | Eggen, André | Brunel, Dominique | Faivre-Rampant, Patricia
Since mid-2005, Illumina Infinium® genotyping arrays provide data with good accuracy for thousands of SNPs (single nucleotide polymorphism) in thousands individuals for many organisms including plants. These extensive data used in large studies gave new insight in population and molecular genetics. Nevertheless, flexible high density genotyping tools at reasonable costs are still to be developed. Low cost genotyping assays would allow many applications including control of individuals, marker assisted management and genetic approaches in species where molecular developments have a comparatively low value relative to the cost of SNP arrays. The initial cost and/or the minimum sample number requirement are limiting factors for developing a new genotyping tool. An add-on option on already existing product gives a first opportunity to reduce the cost of a beadchip. Designing a multi-species array could be another alternative to increase the number of SNPs and decrease the minimum number of samples for each species, reaching lower genotyping cost assay per species.
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