Influence of different concentrations and combinations of cytokinins and auxins on the proliferation of tomato (Lycopersicon esculentum Mill.) callus tissue
2022
In vitro, the effect of culture medium differing in the concentration and combination of plant growth regulators auxins and cytokinins on the callusogenesis and proliferation processes of two tomato cultivars H-2274 (Heinz) and Gardemarin belonging to the (Lycopersicon esculentum Mill.) species was studied. The best indicators of callus formation and proliferation of all three explants used in the H2274 variety were observed when cultured in D variant (4 mg/l kinetin + 1 mg/l indolylacetic acid (IAA) for 8 weeks. Except for option F (BAP + NAA), there were no significant differences in the frequency of callus induction among the remaining 3 media tested. Callus induction and proliferation were lower in this variant than in other variants. Callus induction was poor in all variants tested in cultivar Gardemarin. The cultured callus tissue and nutrient medium in variant M (BAP-4mg/l + 2mg/l 2,4-D) started to darken in the second subcultivation period. Probably, callus tissue and darkening of the medium in variant M can be related to both hormonal content and genotype.
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Este registro bibliográfico ha sido proporcionado por Institute of Molecular Biology and Biotechnologies