Yield stimulation of the rubber tree (RRIM 600) with ethephon does not increase latex Hev b 6 protein or mRNA.
2006
Arokiaraj, P. | Yeang, H.Y.
Hevea brasiliensis latex hevein is an allergenic protein designated the allergen name Hev b 6 by the International Union of Immunological Societies. A study was undertaken to determine if latex flow stimulation by ethephon increased Hev b 6 concentration in the latex from mature rubber trees. A two-site immunoenzymatic assay with antibodies specific for hevein revealed no significant differences in Hev b 6 content in control trees and Hevea trees stimulated with ethephon. The mRNA transcript levels of hevein determined by northern analysis appeared similar for control and stimulated trees. Using the relative ratio method for Real Time PCR assessment, the steady-state transcript levels of hevein were also similar for control and stimulated trees. It was concluded that latex from mature Hevea trees stimulated with ethephon did not result in an increase in hevein mRNA transcripts or in the translated protein. There was therefore no evidence from this study that ethephon stimulation increased allergenicity of latex by increasing Hev b 6 content.
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