Plasmid and antimicrobial resistance transfer from poultry strains to aquatic strains of Escherichia coli
1996
Son, R. | Rusul, G | M. I. A. Karim
Plasmid DNA analysis and antibiotic susceptibility were used to study strains of Escherichia coli isolated from poultry and aquatic sources. Nineteen of 25 and 22 of 30 among the aquatic and poultry strains respectively were found to contain plasmid DNA bands ranging in size from 2.3 to 102 kilobases (kb). Resistances to five or more of the antimicrobial agents tested were predominant among the poultry strains. However, none were susceptible to all antimicrobial agents tested. More isolates containing plasmid were resistant to ampicillin, erythromycin, streptomycin and tetracycline. The potential of the transfer of nature plasmids between poultry and aquatic strains has been studied. Four antibiotic-multiresistant poultry isolates (EP201, EP205, EP210 amd EP211) possessing potentially conjugative large plasmids were mated with two aquatic isolate (EA101 and EA106) and a laboratory strain E. Coli K12 C600 Naʳ in sterilised river water at 37 °C and 25 °C. and LB medium at 37°C. Conjugation occurred in all system tested with en bloc transfer of the donor’s antibiotic resistance and plasmid DNA bands. In invitro transfer conducted at 25°C in sterile river water, transfer frequency values decreased by 2 to 3 logs from the optimum conditions at 37°C.
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